Relevant papers about isomiRs and other novel small RNAs with functional relevance¶
miR-142-3p isomiR: “We furthermore demonstrate that miRNA 5′-end variation leads to differential targeting and can thus broaden the target range of miRNAs.”
A highly expressed miR-101 isomiR is a functional silencing small RNA.
A challenge for miRNA: multiple isomiRs in miRNAomics.
miR-183-5p isomiR changes in breast cancer. Validated target regulation of new genes different from the reference miRNA.
A comprehensive survey of 3’ animal miRNA modification events and a possible role for 3’ adenylation in modulating miRNA targeting effectiveness.
PAPD5-mediated 3′ adenylation and subsequent degradation of miR-21 is disrupted in proliferative disease.
High-resolution analysis of the human retina miRNome reveals isomiR variations and novel microRNAs.
A novel piRNA mechanism in regulating gene expression in highly differentiated somatic cells.
Differential and coherent processing patterns from small RNAs to detect changes in profiles of processing small RNAs.
miRQC: work studying the accuracy and specificity of different technologies to detect miRNAs.
Important features affecting the detection of small RNA biomarkers: How the sample can affect the detection of biomarkers (like RIN value, concentration, ...)
Comparison of alignment and normalization . I will take the message that TMM and DESeq/2 normalization are the best to avoid strong bias if we consider to have a small proportion of DE miRNAs. For the alignments, here you have another comparison for miRNAs annotation: https://rawgit.com/lpantano/tools-mixer/master/mirna/mirannotation/stats.html
review of tools for miRNA de-novo and interaction analysis