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Welcome to seqbuster/seqcluster’s documentation!¶

Analysis of small RNA sequencing data. It detect unit of transcription over the genome, annotate them and create an HTML interactive report that helps to explore the data quickly.

Contents:

  • Installation
    • Seqcluster
    • Tools dependecies for a full small RNA pipeline
    • Data
    • R package
  • Citation
  • Getting started
    • clustering of small RNA sequences
    • Interactive HTML Report
    • Easy start with bcbio-nextgen.py
  • Outputs
    • seqcluster
    • Report
  • Examples of small RNA analysis
    • miRQC data
  • miRNA annotation
    • Processing of reads
    • Prepare databases
    • miRNA/isomiR annotation with JAVA
    • Convert to GFF3-srna
    • Post-analysis with R
    • Manual of miraligner(JAVA)
  • Collapse fastq(.gz) files
  • Handling multi-mapped reads
  • Tools for downstream analysis
    • Web-servers
    • Command-lines
  • Relevant papers about isomiRs and other novel small RNAs with functional relevance
    • Validation
    • IsomiRs
    • General
    • Targets
    • Techonolgy
  • Documentation
  • Classes

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Indices and tables¶

  • Index
  • Module Index
  • Search Page

Navigation

  • Installation
  • Citation
  • Getting started
  • Outputs
  • Examples of small RNA analysis
  • miRNA annotation
  • Collapse fastq(.gz) files
  • Handling multi-mapped reads
  • Tools for downstream analysis
  • Relevant papers about isomiRs and other novel small RNAs with functional relevance
  • Documentation
  • Classes

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